an efficient in vitro propagation, antioxidant and antimicrobial activities of aphyllorchis montana (reichenb.f.)

Authors

ganesan mahendran

abstract

an in vitro plant regeneration protocol was successfully established in aphyllorchis montana , a saprophytic achlorophyllous orchid by culturing immature seeds. among the six basal media evaluated for seed germination, bm-tm medium was found to be the best followed by kc medium. after 40 days, all the media turned brown and the growths of the protocorms were arrested. activated charcoal, 1 g/l in half strength bm-tm was found to be suitable for further development of protocorms. half strength bm-tm medium was supplemented with different growth regulators either individually or in combinations for multiplication of shoots. of the five cytokinins tested, tdz at 6.8 μm was found to be most effective for multiple shoot induction yielding 17.24 ± 0.27 shoots after 10 weeks of culture. addition of low concentration of naa (1.3 μm) in ms medium supplemented with the cytokinin tdz (6.8 μm) favoured shoot multiplication. a mean number of 27.56 ± 0.54 shoots with 3.92 ± 0.11 number of roots were produced per explant. the response of the seed derived protocorm to the different types of organic additives viz., peptone and yeast extract and coconut water was also evaluated. the addition of these organic additives to the medium containing tdz enhanced the number of shoot regeneration. the plantlets were acclimatized in plastic pots containing sterilized vermiculite. the survival rate was 100 % when maintained in the culture room condition (25 ± 2 °c). screening of the antibacterial, antioxidant activity and estimation of total phenolics and flavonoid content of methanolic extracts of micropropagated plants were also carried out and compared with that of the wild-grown plants. in all the tests, methanolic extract from wild-grown plants showed higher antioxidant, antimicrobial activity, total phenolics and flavonoid content than in vitro propagated plants.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

An Efficient In Vitro Propagation, Antioxidant and Antimicrobial Activities of Aphyllorchis Montana (Reichenb.f.)

An in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by KC medium. After 40 days, all the media turned brown and the growths of the protocorms were arrested. Activated charcoal, 1 g/l ...

full text

antioxidant and antimicrobial activity of allium jesdianum extract

چکیده بررسی فعالیت ضد اکسیدانی و ضدمیکروبی عصاره گیاه بن سرخ (allium jesdianum) به کوشش: زهرا مقیمی هدف از این تحقیق بررسی میزان ترکیبات فنولی کل، ترکیبات فلاوونوئیدی کل، فعالیت ضداکسیدانی و ضدمیکروبی عصاره ی گیاه تازه و خشک بن سرخ بود. استخراج عصاره به روش های غوطه وری، استفاده از همزن مغناطیسی، دستگاه مایکروویو، امواج فراصوت و گرمادهی مقاومتی و با استفاده از متانول، اتانول و آب به عنوا...

An Efficient and Cost Effective Protocol for In Vitro Propagation of Pineapple

An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost ...

full text

Antioxidant and Antimicrobial Activities of Essential Oil of Lemon (Citrus limon) Peel in Vitro and in a Food Model

Background: Citrus fruits have some antioxidant and antimicrobial properties. The aim of this study was to determine the chemical compounds, antioxidant, and antimicrobial activities of Essential Oil (EO) of lemon (Citrus limon) peel in vitro and in a food model. Methods: The analysis of the lemon peel EO was carried out using gas chromatography-mass spectrometry. Total phenolic and flavonoid ...

full text

Antioxidant Potential, Lipid Peroxidation Inhibition and Antimicrobial Activities of Satureja montana L. subsp. kitaibelii Extracts

The antioxidant activity of different Satureja montana L. subsp. kitaibelii extracts was tested by measuring their ability to scavenge reactive hydroxyl radical during the Fenton reaction, using ESR spectroscopy. Also, the influence of these extracts on lipid peroxyl radicals obtained during lipid peroxidation of: (I) sunflower oil (37 C, 3h) induced by 4,4'-azobis(4-cyanovaleric acid) (ACVA) a...

full text

Comparison of antioxidant and antibacterial activities of various herbal essential oils: An In vitro study

Background and Aims: Herbal essential oils (EOs) have antimicrobial and antioxidant activities due to the high amount of bioactive compounds; therefore, they are considered good candidates for applications in the food and pharmaceutical industries. The present study aimed to assess the total phenolic content and in vitro comparative study of the biological activities of EOs from different plant...

full text

My Resources

Save resource for easier access later


Journal title:
journal of ornamental plants

Publisher: islamic azad university

ISSN 2251-6433

volume 4

issue 4 2014

Keywords
[ ' a n t i m i c r o b i a l a c t i v i t y ' , ' a n t i o x i d a n t a c t i v i t y ' , ' a p h y l l o r c h i s m o n t a n a ' , ' a s y m b i o t i c s e e d g e r ' , ' m i n a t i o n ' , ' i n v i t r o p r o p a g a t i o n ' , ' p l a n t g r o w t h r e g u l a t o r s ' , 2 , 2 , ' d i p h e n y l ' , 1 , ' p i c r y l h y d r a z y l ' ]

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023